Kesesuaian Uji Imunokromatografi dengan Enzyme Linked Fluorescent Assay untuk Deteksi Hepatitis B Surface Antigen

Abstract

Hepatitis B virus (VHB) infection is a global health problem. Hepatitis B surface antigen (HBsAg) as a serologic marker of hepaitis B virus infection can be detected by immunochromatography and Enzyme Linked Fluorescent Assay (ELFA). The aim of this study is to prove the agreement of immunochromatographic test with ELFA to detect HBsAg. This study used a cross-sectional analytic method on 30 patients for HBsAg examination at the Central Laboratory of Clinical Pathology. HBsAg was tested in patient's serum using immunochromatography and ELFA test. The agreement between the two methods was analyzed using the Kappa test. The Kappa coefficient between the two tests was 0.69 with good strength. The concordance of immunochromatographic assay with ELFA to detect HBsAg in this study gave good results.